Formation of recombinant DNA requires a cloning vector, a DNA molecule that replicates within a living cell. … The choice of vector for molecular cloning depends on the choice of host organism, the size of the DNA to be cloned, and whether and how the foreign DNA is to be expressed.
What are recombinant DNA molecules?
Recombinant DNA includes molecules constructed outside of living cells by joining natural or synthetic DNA segments to DNA molecules that can replicate in a living cell, or molecules that result from their replication.
What is an example of DNA recombination?
For example, insulin is regularly produced by means of recombinant DNA within bacteria. A human insulin gene is introduced into a plasmid, which is then introduced to a bacterial cell. The bacteria will then use its cellular machinery to produce the protein insulin, which can be collected and distributed to patients.